Simplified technique for detection of significant bacteriuria by microscopic examination of urine

J Clin Microbiol. 1998 Mar;36(3):820-3. doi: 10.1128/JCM.36.3.820-823.1998.

Abstract

A comparative study of microscopic examination of 10 microl (simplified loop technique) and 50 microl (traditional drop technique) of uncentrifuged Gram-stained urine specimens for detection of significant bacteriuria was carried out. The results demonstrated that the 10-microl loop technique can be used as an alternative to the 50-microl drop technique for presumptive diagnosis of urinary-tract infection in bacteriological practice, with the advantages of greater rapidity and ease of performance.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques*
  • Bacteriuria / diagnosis*
  • Gentian Violet
  • Humans
  • Phenazines
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Staining and Labeling
  • Urine / microbiology*

Substances

  • Gram's stain
  • Phenazines
  • Gentian Violet