The coactivation of hamstrings and quadriceps, and its relation to knee joint stability and cruciate ligament loading, have been extensively examined over the last decades. The purpose of this review is to present findings on the quantification of antagonist activation around the knee. Coactivation of the quadriceps and hamstrings during many activities has been examined using electromyography (EMG). However, there are several factors that affect antagonist EMG activity, such as the type of muscle action, velocity of the movement, level of effort and angular position. Furthermore, the antagonist EMG can be affected by methodological factors which relate to the data recording, analysis and quantification of the signal. Research has demonstrated that the effect of cross-talk on the hamstrings and quadriceps antagonist EMG depends on electrode size and location, fat layer of the muscles and the technique used to reduce it. There is an inconsistency as to the method used to normalise antagonist EMG depending on the type of movement examined. This makes comparisons between studies difficult and, therefore, further research is recommended. The antagonist function is better represented when the antagonist moment exerted is known; however, the direct measurement of antagonist moments or forces is very difficult. Few studies have used mathematical models to determine the moment or force distribution around the knee, including antagonist forces. This can be attributed to the complexity of the anatomy and function of the knee joint. Despite this, in vivo and in vitro experiments have demonstrated that quadriceps contraction near full extension induces significantly higher anterior shear forces when compared with the forces exerted when the hamstrings act as antagonists, thus indicating the important role of antagonist activity in knee joint stability. However, the magnitude of this contribution to the force distribution around the knee in many activities is unclear.