The purpose of this study is to investigate the effects of repeated rotating stimuli on electronystagmogram (ENG) and electrogastrogram (EGG) in guinea pigs. ENG and EGG were separately recorded prior to the stimuli and on the next day and 30 days after repeated stimuli in 14 guinea pigs with normal pinna reflexes. The results showed that the numbers of the bilateral vestibular nystagmus (L and R separately represented the numbers of left and right nystagmus) of postrotation (L = 682, R = 657) were obviously less than that of prerotation (L = 857, R = 842) (P < 0.01, pairing t-test), and EGG amplitude of postrotation (4506 microV) during the whole course of rotating stimuli was lower than that of prerotation (5279 microV) (P < 0.01, pairing t-test). The numbers of bilateral nystagmus (L = 845, R = 826) were obviously raised again 30 days after stopping rotating stimuli (P < 0.01, compared with next day after stopping repeated stimuli; P > 0.05 compared with before rotating stimuli). It was concluded that repeated rotating stimuli can decrease the vestibulo-ocular and vestibulo autonomic reflexes in guinea pigs, but the effects were not timeless.