Sequence completion, identification and definition of the fengycin operon in Bacillus subtilis 168

Microbiology (Reading). 1997 Nov:143 ( Pt 11):3443-3450. doi: 10.1099/00221287-143-11-3443.

Abstract

A 15 kb DNA fragment from the Bacillus subtilis chromosome between citB and ppsC has been sequenced, and new ORFs encoding putative enzymes involved in lipopolypeptide synthesis, which complete a partial operon previously reported, and a new set of enzymes responsible for lipid metabolism have been identified. From the analysis of DNA sequence homology of the fragment it was deduced that these new peptide synthetase genes are part of an operon for the biosynthesis of the fungicide fengycin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / biosynthesis*
  • Bacillus subtilis / enzymology
  • Bacillus subtilis / genetics*
  • Fatty Acids / metabolism
  • Genes, Bacterial / genetics*
  • Lipopeptides
  • Lipoproteins / biosynthesis
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics
  • Open Reading Frames / genetics
  • Operon / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Antifungal Agents
  • Fatty Acids
  • Lipopeptides
  • Lipoproteins
  • Multienzyme Complexes
  • fengycin

Associated data

  • GENBANK/Y13917