Ribotyping for strain characterization of Clostridium perfringens isolates from food poisoning cases and outbreaks

B Schalch; J Björkroth; H Eisgruber; H Korkeala; A Stolle

doi:10.1128/aem.63.10.3992-3994.1997

Ribotyping for strain characterization of Clostridium perfringens isolates from food poisoning cases and outbreaks

Appl Environ Microbiol. 1997 Oct;63(10):3992-4. doi: 10.1128/aem.63.10.3992-3994.1997.

Authors

B Schalch¹, J Björkroth, H Eisgruber, H Korkeala, A Stolle

Affiliation

¹ Institute for Hygiene and Technology of Foods of Animal Origin, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany.

Abstract

Ribotyping was used to characterize 34 Clostridium perfringens strains isolated from 10 food poisoning cases and outbreaks over a 7-year period. Twelve different ribopatterns were generated by EcoRI digestion. In eight food poisoning cases and outbreaks, all of the ribotypes of each food and stool isolate were found to be identical. Two C. perfringens isolates showed unique patterns. Ribotyping was found to be a useful tool for determining the genetic relationship of C. perfringens isolates in the context of foodborne poisoning cases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Typing Techniques
Clostridium Infections / epidemiology
Clostridium Infections / microbiology*
Clostridium perfringens / classification*
Clostridium perfringens / genetics*
Clostridium perfringens / isolation & purification
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Deoxyribonuclease EcoRI
Disease Outbreaks
Feces / microbiology
Food Microbiology
Foodborne Diseases / epidemiology
Foodborne Diseases / microbiology*
Germany / epidemiology
Humans

Substances

DNA, Bacterial
Deoxyribonuclease EcoRI