Problem: To investigate the role of the plasma cofactor for antiphospholipid antibodies in antibody binding to endothelial cells.
Method of study: a) Evaluation of endothelial cell binding of polyclonal and monoclonal anti-beta 2 glycoprotein I antibodies; and b) study of the effects of antibody binding: adhesion molecule expression, leukocyte adhesion, and interleukin-1 beta secretion.
Results: Anti-beta 2 glycoprotein I antibodies bind endothelial cell monolayers in vitro by reacting with the cofactor adhered to the cell membranes. Antibody binding induces an up-regulation of adhesion molecules, favours leukocyte adherence, and increases interleukin-1 beta secretion. Interleukin-1 beta plays an active role to mediate adhesion molecule expression through an autocrine loop, as shown by the inhibitory effect of interleukin 1 receptor antagonist.
Conclusions: Antiphospholipid antibodies do react with endothelium through the co-factor adhered to their cell membranes and induces a pro-adhesive cell phenotype.