Abstract
The fine specificities of MAbs generated using novel synthetic clustered STn and Tn glycopeptides as immunogens were compared with the anti-TAG-72 antibodies B72.3 and CC49. Hapten inhibition experiments demonstrated the specificity of several of the MAbs for STn and Tn expressed on ovine submaxillary mucin and tumor derived MUC-1 mucin. Amongst the STn specific MAbs only the B195.3 MAb shows absolute dependence on the presence of sialic acid and specificity to the simple disaccharide NANAA alpha2-6-GalNAc. Identification of tumor associated carbohydrate epitopes in cluster and monomer configurations are possible using MAbs detecting the defined structure specificities described herein.
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Antibody Specificity
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Antigens, Tumor-Associated, Carbohydrate / analysis*
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Antigens, Tumor-Associated, Carbohydrate / immunology
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Ascitic Fluid / chemistry
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Biomarkers, Tumor
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Breast Neoplasms / chemistry
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Carbohydrate Conformation
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Carbohydrate Sequence
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Enzyme-Linked Immunosorbent Assay
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Epitopes / analysis
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Female
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Glycopeptides / analysis
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Glycopeptides / chemical synthesis
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Glycosylation
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Haptens
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Mice
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Molecular Sequence Data
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Mucin-1 / biosynthesis
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Mucin-1 / chemistry
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Mucins / biosynthesis
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Mucins / chemistry
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N-Acetylneuraminic Acid / analysis
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Radioimmunoassay
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Sheep
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Submandibular Gland / metabolism
Substances
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Antibodies, Monoclonal
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Antigens, Tumor-Associated, Carbohydrate
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Biomarkers, Tumor
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Epitopes
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Glycopeptides
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Haptens
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Mucin-1
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Mucins
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Tn antigen
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sialosyl-Tn antigen
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N-Acetylneuraminic Acid