Specificities of anti-sialyl-Tn and anti-Tn monoclonal antibodies generated using novel clustered synthetic glycopeptide epitopes

Glycoconj J. 1997 Aug;14(5):549-60. doi: 10.1023/a:1018576224062.

Abstract

The fine specificities of MAbs generated using novel synthetic clustered STn and Tn glycopeptides as immunogens were compared with the anti-TAG-72 antibodies B72.3 and CC49. Hapten inhibition experiments demonstrated the specificity of several of the MAbs for STn and Tn expressed on ovine submaxillary mucin and tumor derived MUC-1 mucin. Amongst the STn specific MAbs only the B195.3 MAb shows absolute dependence on the presence of sialic acid and specificity to the simple disaccharide NANAA alpha2-6-GalNAc. Identification of tumor associated carbohydrate epitopes in cluster and monomer configurations are possible using MAbs detecting the defined structure specificities described herein.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibody Specificity
  • Antigens, Tumor-Associated, Carbohydrate / analysis*
  • Antigens, Tumor-Associated, Carbohydrate / immunology
  • Ascitic Fluid / chemistry
  • Biomarkers, Tumor
  • Breast Neoplasms / chemistry
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis
  • Female
  • Glycopeptides / analysis
  • Glycopeptides / chemical synthesis
  • Glycosylation
  • Haptens
  • Mice
  • Molecular Sequence Data
  • Mucin-1 / biosynthesis
  • Mucin-1 / chemistry
  • Mucins / biosynthesis
  • Mucins / chemistry
  • N-Acetylneuraminic Acid / analysis
  • Radioimmunoassay
  • Sheep
  • Submandibular Gland / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, Tumor-Associated, Carbohydrate
  • Biomarkers, Tumor
  • Epitopes
  • Glycopeptides
  • Haptens
  • Mucin-1
  • Mucins
  • Tn antigen
  • sialosyl-Tn antigen
  • N-Acetylneuraminic Acid