Abstract
Evidence is accumulating suggesting that platelet-activating factor plays a role in inflammatory dermatoses. Mass spectrometric methods were used to examine the molecular species of sn-2 acetyl glycerophosphocholines (GPC) synthesized by primary cultures of human neonatal foreskin-derived keratinocytes. Ionophore-stimulated keratinocytes synthesize both 1-alkyl and 1-acyl sn-2 acetyl-GPC, and the relative amounts were as follows: hexadecyl > palmitoyl > octadecyl > stearoyl at the sn-1 position. PAF synthesis in the keratinocyte-derived cell line HaCaT was inhibited by dexamethasone, suggesting that the anti-inflammatory effects of glucocorticosteroids in inflammatory dermatoses might be in part related to the inhibition of the synthesis of mediators such as PAF.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adrenal Cortex Hormones / metabolism
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Anti-Inflammatory Agents / pharmacology
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Arachidonic Acid / metabolism
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Calcimycin / pharmacology
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Cell Line
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Dexamethasone / pharmacology
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Humans
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Infant, Newborn
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Ionophores / pharmacology
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Keratinocytes / chemistry
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Keratinocytes / metabolism*
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Kinetics
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Mass Spectrometry
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Platelet Activating Factor / analogs & derivatives*
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Platelet Activating Factor / analysis
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Platelet Activating Factor / antagonists & inhibitors
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Platelet Activating Factor / biosynthesis*
Substances
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1-acyl-2-acetyl-sn-glycero-3-phosphocholine
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Adrenal Cortex Hormones
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Anti-Inflammatory Agents
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Ionophores
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Platelet Activating Factor
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Arachidonic Acid
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Calcimycin
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Dexamethasone