Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel

A Eldar; S Lawhon; P F Frelier; L Assenta; B R Simpson; P W Varner; H Bercovier

doi:10.1111/j.1574-6968.1997.tb12564.x

Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel

FEMS Microbiol Lett. 1997 Jun 15;151(2):155-62. doi: 10.1111/j.1574-6968.1997.tb12564.x.

Authors

A Eldar¹, S Lawhon, P F Frelier, L Assenta, B R Simpson, P W Varner, H Bercovier

Affiliation

¹ Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

PMID: 9228748
DOI: 10.1111/j.1574-6968.1997.tb12564.x

Abstract

Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178T (T = Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA, Ribosomal / analysis*
Israel
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S / genetics*
Streptococcus / classification*
Streptococcus / genetics
United States

Substances

DNA, Ribosomal
RNA, Ribosomal, 16S