A simple method for the purification of type 1 RIPs

Biomed Chromatogr. 1997 May-Jun;11(3):151-3. doi: 10.1002/(SICI)1099-0801(199705)11:3<151::AID-BMC651>3.0.CO;2-S.

Abstract

We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Ion Exchange
  • Electrophoresis, Polyacrylamide Gel
  • Isoelectric Point
  • N-Glycosyl Hydrolases / isolation & purification*
  • Plant Proteins / isolation & purification*
  • Plant Roots / chemistry
  • Plant Roots / enzymology*
  • Ribosome Inactivating Proteins, Type 2
  • Seeds / chemistry
  • Seeds / enzymology*
  • Silver Staining

Substances

  • Plant Proteins
  • Ribosome Inactivating Proteins, Type 2
  • N-Glycosyl Hydrolases