Abstract
We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, Ion Exchange
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Electrophoresis, Polyacrylamide Gel
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Isoelectric Point
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N-Glycosyl Hydrolases / isolation & purification*
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Plant Proteins / isolation & purification*
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Plant Roots / chemistry
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Plant Roots / enzymology*
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Ribosome Inactivating Proteins, Type 2
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Seeds / chemistry
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Seeds / enzymology*
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Silver Staining
Substances
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Plant Proteins
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Ribosome Inactivating Proteins, Type 2
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N-Glycosyl Hydrolases