In laboratory experiments, Aedes albopictus females were fed hepatitis C virus (HCV)-infected human blood with a unit-feeding apparatus. Pools of engorged females were tested at various time intervals after their blood meal for the presence of HCV RNA using two methods in parallel: reverse transcription-nested polymerase chain reaction (PCR) and PCR with the Amplicor HCV system. Comparable results were obtained with both methods. Mosquitoes were found to be positive only immediately and 24 h after feeding. Moreover, female progeny produced negative results. Our results indicate that the Ae.albopictus strain used is incompetent as a vector of HCV.