Production of a T-cell clone which reacts with membrane proteins of Acholeplasma laidlawii

Microbiol Immunol. 1997;41(3):261-4. doi: 10.1111/j.1348-0421.1997.tb01198.x.

Abstract

The role of cellular immunity in mycoplasma infection is not completely understood. In this study, we established mycoplasma-specific T-cell clones to evaluate cellular immunity in mycoplasma infection. We developed a T-cell clone (G-10) which was stimulated with Acholeplasma laidlawii. The T-cell clone G-10, CD4+ and T-cell receptor (TCR) alpha beta- recognized the 42- and 65-kilodalton (kDa) membrane proteins of A. laidlawii and responded to A. hippikon. Hence, the application of mycoplasma-specific T cells such as G-10 in animal models may allow the assessment of cellular immune response to mycoplasma infection.

Publication types

  • Comparative Study

MeSH terms

  • Acholeplasma laidlawii / immunology*
  • Animals
  • Antigen-Presenting Cells
  • Bacterial Proteins / immunology*
  • Clone Cells
  • Cross Reactions
  • H-2 Antigens
  • Immunity, Cellular
  • Lymphocyte Activation
  • Major Histocompatibility Complex
  • Membrane Proteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Species Specificity
  • Spleen / cytology
  • Spleen / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*

Substances

  • Bacterial Proteins
  • H-2 Antigens
  • Membrane Proteins