Abstract
An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and late gene expression was observed in noncomplementing A549 cells, and a reduction in viral growth was seen in the E1-expressing 293 cells. In contrast, there was no repression in the presence of the regulator tetracycline. We propose that regulation by TetR-KRAB is a valuable tool with which to study the effects of viral gene expression in vitro.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenoviridae / genetics*
-
Adenovirus E1 Proteins / genetics*
-
Adenovirus E2 Proteins / genetics*
-
Adenovirus E3 Proteins / genetics*
-
Capsid / genetics
-
Capsid Proteins*
-
Cell Line, Transformed
-
Cloning, Molecular
-
DNA Replication
-
DNA, Viral / biosynthesis*
-
DNA-Binding Proteins / genetics
-
Gene Deletion
-
Gene Expression Regulation, Viral*
-
Genetic Complementation Test
-
Genetic Vectors*
-
Humans
-
Kruppel-Like Transcription Factors
-
Repressor Proteins / genetics
-
Transcription Factors / genetics
-
Transcription, Genetic
-
Tumor Cells, Cultured
-
Viral Structural Proteins
Substances
-
Adenovirus E1 Proteins
-
Adenovirus E2 Proteins
-
Adenovirus E3 Proteins
-
Capsid Proteins
-
DNA, Viral
-
DNA-Binding Proteins
-
Kruppel-Like Transcription Factors
-
Repressor Proteins
-
Transcription Factors
-
Viral Structural Proteins
-
hexon capsid protein, Adenovirus
-
penton protein, adenovirus
-
tetracycline resistance-encoding transposon repressor protein