Presence of paf-acether in human blood after thin-layer chromatography, but not after high-performance liquid chromatography purification

J Pharmacol Toxicol Methods. 1996 Dec;36(4):219-21. doi: 10.1016/s1056-8719(96)00129-3.

Abstract

After HPLC purification of human blood extracts, paf-acether (paf) was found exclusively as a lipoprotein-bound compound (lipopaf), whereas free-paf was absent. When the same samples (or lipopaf recovered from HPLC) were purified by TLC, both free-paf and lipopaf were detected. The free-paf detected in blood samples could thus result from lipopaf dissociation during TLC purification.

Publication types

  • Comparative Study

MeSH terms

  • Blood Chemical Analysis / methods*
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Humans
  • Lipoproteins / analysis
  • Lipoproteins / metabolism
  • Platelet Activating Factor / analysis*
  • Platelet Activating Factor / metabolism

Substances

  • Lipoproteins
  • Platelet Activating Factor