Abstract
The yeast two-hybrid screening was applied to cloning cDNAs of proteins that interact with peroxisome proliferator-activated receptor alpha (PPAR alpha). We obtained from a rat liver cDNA library a clone encoding a protein related to the ligand-binding domain of the members of nuclear hormone receptor superfamily, whereas apparently lacking the zinc-finger DNA-binding domain. This protein interacted with the activated forms of several nuclear receptors, and thus is a novel type of heterodimer-forming nuclear receptor.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA, Complementary
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Library
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Humans
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Liver / metabolism*
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Molecular Sequence Data
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Organ Specificity
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Rats
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Receptors, Cytoplasmic and Nuclear / chemistry
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Restriction Mapping
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Zinc Fingers
Substances
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DNA, Complementary
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DNA-Binding Proteins
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Receptors, Cytoplasmic and Nuclear
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Recombinant Proteins
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Transcription Factors
Associated data
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GENBANK/D86580
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GENBANK/D86745
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GENBANK/D86839