Abstract
Cattle were successfully immunized against heartwater with a lysate of Cowdria ruminantium formulated in Freund's adjuvant. Vaccinated animals proved fully resistant to virulent challenge 3 and 10 months after vaccination. For the first time a helper T lymphocyte response to Cowdria antigens was observed and characterized. Cowdria-specific T-cell lines generated from vaccinated animals by in vitro restimulation with Cowdria lysates are 95 to 100% CD4+, are MHC class II restricted, and produce gamma interferon. They proliferate in response to autologous monocytes infected with live Cowdria but not in response to uninfected monocytes. These T-cell lines will facilitate the search for Cowdria antigens that are immunogenic for T cells and will therefore be of relevance in the development of a subunit vaccine against the disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Bacterial / therapeutic use
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Bacterial Vaccines / therapeutic use*
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology*
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / prevention & control*
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Cell Line
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Flow Cytometry
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Freund's Adjuvant
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Heartwater Disease / immunology
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Heartwater Disease / mortality
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Heartwater Disease / prevention & control*
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Histocompatibility Antigens Class II / immunology
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Interferon-gamma / analysis
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Lymphocyte Activation
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Major Histocompatibility Complex / immunology
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Subcellular Fractions / immunology
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Vaccination*
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Vaccines, Inactivated / therapeutic use*
Substances
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Antigens, Bacterial
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Bacterial Vaccines
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Histocompatibility Antigens Class II
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Vaccines, Inactivated
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Interferon-gamma
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Freund's Adjuvant