To identify genes differentially expressed in the developing rat cerebellum, a subtractive cDNA cloning procedure, followed by differential screening, was used. Four novel genes, MB, MF, 3E7, and 3C6, the transcriptional activity of which changed by a factor of five during cerebellar development, were isolated. The genes obtained were differentially expressed in different regions of the central nervous system. Variations in the levels of corresponding transcripts in the brain cortex and cerebellum were also recorded during postnatal rat development.