Abstract
Pre-treatment with the immunosuppressant FK506 is shown to protect primary cardiocytes against a subsequent severe thermal or ischaemic stress. This effect is not observed with the related compounds cyclosporin A or rapamycin. It does not involve induction of the FK506 binding, heat inducible protein hsp56 or of the other heat shock proteins. In addition over-expression of hsp56 does not protect cardiac cells from severe stress in contrast to our previous results with hsp70 and hsp90. These results suggest the FK506 is acting via a novel mechanism to protect cardiac cells against cellular ischaemia which may not be related to its immunosuppressant action.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / metabolism
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Animals
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Animals, Newborn
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Blotting, Western
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Carrier Proteins / metabolism
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Cell Line
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Cell Survival
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Cyclosporine / administration & dosage
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DNA-Binding Proteins / metabolism
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Dose-Response Relationship, Drug
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Electrophoresis, Polyacrylamide Gel
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HSP70 Heat-Shock Proteins / metabolism
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HSP90 Heat-Shock Proteins / metabolism
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Heat-Shock Proteins / metabolism
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Immunosuppressive Agents / administration & dosage*
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Ischemic Preconditioning, Myocardial / methods*
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Myocardium / metabolism*
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Polyenes / administration & dosage
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Rats
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Rats, Sprague-Dawley
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Sirolimus
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Stress, Physiological / metabolism
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Stress, Physiological / prevention & control*
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Tacrolimus / administration & dosage*
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Tacrolimus Binding Proteins
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Trypan Blue
Substances
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Carrier Proteins
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DNA-Binding Proteins
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HSP70 Heat-Shock Proteins
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HSP90 Heat-Shock Proteins
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Heat-Shock Proteins
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Immunosuppressive Agents
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Polyenes
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Cyclosporine
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Alkaline Phosphatase
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Tacrolimus Binding Proteins
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Trypan Blue
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Sirolimus
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Tacrolimus