Abstract
PMP-D2, a novel 35 amino acid peptide isolated from the brain of the locust Locusta migratoria, is localised specifically in neurosecretory cells and nerve tracts of the Pars intercerebralis. When PMP-D2 is applied onto rat sensory neurones it blocks high voltage-activated inward Ca2+ currents at concentrations ranging from 0.1 mu M to 10 mu M. The inhibitory effect of PMP-D2 is more marked on the sustained inward Ca2+ current measured at the end of 100 ms voltage step commands than on the maximum inward Ca2+ current. These results suggests that PMP-D2 may differentially inhibit the two components of the high voltage-activated inward Ca2+ currents of rat sensory neurones.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / metabolism*
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Calcium Channels / drug effects
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Calcium Channels / metabolism
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Cyclotides*
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Grasshoppers / metabolism
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Immunohistochemistry
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In Vitro Techniques
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Insect Hormones / isolation & purification
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Insect Hormones / metabolism
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Insect Hormones / pharmacology*
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Insect Proteins*
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Kinetics
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Nerve Tissue Proteins / isolation & purification
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Nerve Tissue Proteins / metabolism
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Nerve Tissue Proteins / pharmacology
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Neurons, Afferent / drug effects*
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Neurons, Afferent / metabolism*
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Rats
Substances
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Calcium Channels
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Cyclotides
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Insect Hormones
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Insect Proteins
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Nerve Tissue Proteins
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PMP-D2 peptide, insect
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Calcium