A novel strategy for O6-protection of guanosine

Nucleic Acids Symp Ser. 1995:(34):51-2.

Abstract

A "one-pot reaction" has been used for simultaneous protection of amino and O6-function of deoxyguanosine. N-(4-bromobutyl) phthalimide used for O6-protection generates an alkylamino function after mild base hydrolysis. Two sequences d(GGA) and d(GGATCC) have been synthesised with all protected guanosine units. Similarly, 2-(vinyl)pyridine has also been used for O6-protection following the same strategy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Deoxyguanosine / chemical synthesis
  • Deoxyguanosine / chemistry
  • Guanosine / chemical synthesis*
  • Guanosine / chemistry*
  • Indicators and Reagents
  • Methods
  • Molecular Structure
  • Oligodeoxyribonucleotides / chemical synthesis
  • Oligodeoxyribonucleotides / chemistry
  • Phthalimides
  • Pyridines

Substances

  • Indicators and Reagents
  • Oligodeoxyribonucleotides
  • Phthalimides
  • Pyridines
  • Guanosine
  • 2-vinylpyridine
  • Deoxyguanosine