Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting

FEMS Microbiol Lett. 1996 Oct 1;143(2-3):259-65. doi: 10.1111/j.1574-6968.1996.tb08490.x.

Abstract

We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates was investigated analyzing hydrophobic membrane protein fractions by immunoblotting using pooled sheep antiserum from naturally infected sheep. Large restriction fragments obtained cleaving genomic DNAs with SmaI, NruI, SalI, XhoI, BssHII and KpnI were analyzed by pulsed field gel electrophoresis. Genetic analysis indicates that isolates are all similar without intraspecific differences. This homogeneity was confirmed by immunoblotting: 80 and 50 kDa antigens are present in all strains analyzed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigenic Variation
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / isolation & purification
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Bacterial Proteins / isolation & purification
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Electrophoresis, Gel, Pulsed-Field
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Immunoblotting
  • Italy / epidemiology
  • Molecular Epidemiology
  • Mycoplasma / genetics*
  • Mycoplasma / immunology*
  • Mycoplasma / isolation & purification
  • Mycoplasma Infections / epidemiology
  • Mycoplasma Infections / microbiology
  • Mycoplasma Infections / veterinary
  • Sheep
  • Sheep Diseases / epidemiology
  • Sheep Diseases / microbiology

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA Restriction Enzymes