Molecular cloning of a genomic DNA for enolase from Aspergillus oryzae

Biosci Biotechnol Biochem. 1996 Jan;60(1):161-3. doi: 10.1271/bbb.60.161.

Abstract

We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5'- and 3'- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.

MeSH terms

  • Amino Acid Sequence
  • Aspergillus oryzae / enzymology*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Bacterial / chemistry*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Enzymologic / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phosphopyruvate Hydratase / chemistry
  • Phosphopyruvate Hydratase / genetics*
  • Restriction Mapping
  • TATA Box

Substances

  • DNA, Bacterial
  • Phosphopyruvate Hydratase

Associated data

  • GENBANK/D63941