Abstract
The immunoglobulin A-specific reactivities of recombinant viral proteins from nine different reading frames of human cytomegalovirus were evaluated in enzyme-linked immunosorbent assay experiments. Antigen fragments of reading frames pUL32, pUL44, and pUL57 were identified as preferable antigens for immunoglobulin A serodiagnosis. Application of autologous fusion proteins which combine these polypeptides may be useful especially for the early detection of acute secondary human cytomegalovirus infection.
MeSH terms
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Acute Disease
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Antibodies, Viral / blood*
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Antigens, Viral* / genetics
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Cytomegalovirus / genetics
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Cytomegalovirus / immunology*
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Cytomegalovirus Infections / diagnosis*
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Cytomegalovirus Infections / immunology
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Enzyme-Linked Immunosorbent Assay / methods
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Evaluation Studies as Topic
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Humans
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Immunoglobulin A / blood*
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Reading Frames
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Serologic Tests / methods*
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Time Factors
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunoglobulin A
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Recombinant Fusion Proteins