Abstract
Heat treatment of Salmonella typhimurium results in cell death, which coincides with a significant reduction of the cellular content of 16S ribosomal RNA. It is suggested that the degradation of ribosomal RNA is a direct cause of cell death. This conclusion is based on the observation of carbon-starved and magnesium-supplemented cells, which survive heat treatment much better, and which also maintain stable levels of ribosomal RNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbon / metabolism
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Cell Death*
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DNA Probes / genetics
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Hot Temperature / adverse effects*
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In Situ Hybridization
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Magnesium / metabolism
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RNA, Ribosomal, 16S / genetics
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RNA, Ribosomal, 16S / metabolism*
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RNA, Ribosomal, 23S / genetics
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RNA, Ribosomal, 23S / metabolism*
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Salmonella typhimurium / metabolism*
Substances
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DNA Probes
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RNA, Ribosomal, 16S
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RNA, Ribosomal, 23S
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Carbon
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Magnesium