Abstract
Chimeric mice in which lymphocytes are deficient in the Syk tyrosine kinase have been created. Compared with Syk-positive controls, mice with Syk -/- lymphocytes display substantial depletion of intraepithelial gamma delta T cells in the skin and gut, with developmental arrest occurring after antigen receptor gene rearrangement. In this dependence on Syk, subsets of intraepithelial gamma delta T cells are similar to B cells, but distinct from splenic gamma delta T cells that develop and expand in Syk-deficient mice. The characteristic associations of certain T-cell receptor V gamma/V delta gene rearrangements with specific epithelia are also disrupted by Syk deficiency.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Electrophoresis, Polyacrylamide Gel
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Enzyme Precursors / genetics*
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Enzyme Precursors / metabolism
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Epithelial Cells
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Epithelium / metabolism
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Flow Cytometry
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Intracellular Signaling Peptides and Proteins
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Lymphocytes / enzymology
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Lymphocytes / immunology
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Lymphocytes / metabolism
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Mice
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Mutagenesis, Site-Directed
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Protein-Tyrosine Kinases / genetics*
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Protein-Tyrosine Kinases / metabolism
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Receptors, Antigen, T-Cell, gamma-delta / genetics*
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Receptors, Antigen, T-Cell, gamma-delta / metabolism
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Syk Kinase
Substances
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Enzyme Precursors
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Intracellular Signaling Peptides and Proteins
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Receptors, Antigen, T-Cell, gamma-delta
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Protein-Tyrosine Kinases
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Syk Kinase
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Syk protein, mouse