Characterisation of vha26, the Drosophila gene for a 26 kDa E-subunit of the vacuolar ATPase

Biochim Biophys Acta. 1996 Aug 14;1283(1):4-9. doi: 10.1016/0005-2736(96)00103-4.

Abstract

A Drosophila melanogaster gene and cDNA for the E-subunit of the V-ATPase were characterised. The deduced product has 226 amino acids and a molecular mass of 26.1 kDa. The gene is a single copy at 83B1-4 on chromosome 3R. The coding sequence is punctuated by three introns which do not align with those in Neurospora. The gene is ubiquitously expressed as an mRNA of 2.3 kb. but at lower levels in pupae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • DNA, Complementary
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics*
  • Gene Expression
  • Molecular Sequence Data
  • Molecular Weight
  • Proton-Translocating ATPases / chemistry
  • Proton-Translocating ATPases / genetics*
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Vacuolar Proton-Translocating ATPases*

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Vacuolar Proton-Translocating ATPases
  • Proton-Translocating ATPases

Associated data

  • GENBANK/M60663
  • GENBANK/P11019
  • GENBANK/P31402
  • GENBANK/P36543
  • GENBANK/U13841
  • GENBANK/U17641
  • GENBANK/U38198
  • GENBANK/U38951
  • GENBANK/X76913
  • GENBANK/X79516
  • GENBANK/X92117
  • GENBANK/X92118
  • GENBANK/X96785