Abstract
Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma virus-specific antibodies in rabbits after immunization. Inoculations by the intradermal route protected animals against virulent RHDV and myxoma virus challenges.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caliciviridae Infections / prevention & control*
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DNA, Recombinant / therapeutic use*
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DNA, Viral / genetics
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Hemorrhagic Disease Virus, Rabbit / genetics*
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Hemorrhagic Disease Virus, Rabbit / immunology
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Hemorrhagic Disease Virus, Rabbit / metabolism
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Myxoma virus / genetics*
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Myxoma virus / immunology
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Myxoma virus / metabolism
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Myxomatosis, Infectious / prevention & control*
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Rabbits
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Reassortant Viruses / genetics*
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Reassortant Viruses / immunology
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Vaccines, Synthetic / therapeutic use*
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Viral Structural Proteins / genetics*
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Viral Structural Proteins / immunology
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Viral Structural Proteins / metabolism
Substances
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DNA, Recombinant
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DNA, Viral
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Vaccines, Synthetic
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Viral Structural Proteins
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viral protein 60, rabbit hemorrhagic disease virus