Differentiation of African horse sickness viruses by polymerase chain reaction and segments 10 restriction patterns

Vet Microbiol. 1995 Dec;47(3-4):365-75. doi: 10.1016/0378-1135(95)00104-2.

Abstract

This paper describes a single tube reverse transcription-polymerase chain reaction (RT-PCR) method for detection of African horse sickness virus (AHSV). The genomic segments 10 of viruses of the 9 AHSV serotypes were amplified. The 758bp products were digested to completion by restriction enzymes. The restriction fragment length polymorphisms of segments 7 and 10 cDNA allowed the differentiation of the nine serotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • DNA, Complementary
  • Horse Diseases*
  • Horses
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Polymorphism, Restriction Fragment Length
  • Reoviridae / classification
  • Reoviridae / genetics*
  • Reoviridae / isolation & purification
  • Reoviridae Infections / diagnosis
  • Reoviridae Infections / veterinary*
  • Reoviridae Infections / virology
  • Restriction Mapping

Substances

  • DNA Primers
  • DNA, Complementary