The N-terminal 303 amino acids of the human cytomegalovirus envelope glycoprotein B (UL55) and the exon 4 region of the major immediate early protein 1 (UL123) induce a cytotoxic T-cell response

Vaccine. 1996 Apr;14(5):369-74. doi: 10.1016/0264-410x(95)00227-r.

Abstract

We reported earlier that an adenovirus (Ad) recombinant expressing the full-length human cytomegalovirus (HCMV) glycoprotein B (gB) gene induces gB-specific cytotoxic T lymphocyte (CTL) responses in CBA (H-2k) mice (Berencsi et al., J. Gen. Virol. 74, 257-2512, 1993). Here we show that mice immunized with Ad recombinant viruses expressing truncated forms of the gB gene containing the first 700 (Ad-700), 465 (Ad-465) or 303 (Ad-303) amino acids of gB or an Ad construct containing exon 4 (E4) of the HCMV immediate early 1 (IEI) gene (Ad-IEI (E4)) demonstrate HCMV-specific CTL responses. These data suggest the importance of the first 303 amino acids of the gB polypeptide and the IEI E4 product in designing a vaccine to induce anti-HCMV CTL responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antigens, Viral / immunology*
  • Cytomegalovirus / immunology*
  • Exons*
  • Female
  • Immediate-Early Proteins / immunology*
  • Mice
  • Mice, Inbred CBA
  • Peptide Fragments / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Immediate-Early Proteins
  • Peptide Fragments
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus
  • immediate-early proteins, cytomegalovirus