Although detection thresholds for odors are commonly measured in academic and medical settings, the influences of procedural factors on threshold values are poorly understood. The present study evaluated the influences of (i) trial sequence position and (ii) diluent type on the threshold value for the rose-like odorant phenyl ethyl alcohol. In Exp. 1, detection thresholds were measured in 24 subjects on two occasions in which different diluents were used in the concentration series, propylene glycol and light mineral oil. The thresholds were estimated using a 7-reversal initially ascending single-staircase procedure. Threshold values were significantly influenced by the type of diluent (lower for mineral oil) and trial sequence (lower for later threshold reversals). In Exp. 2, 24 subjects were administered a staircase threshold test which continued through 15 staircase reversals. Continued testing resulted in a significant lowering of the threshold measure. These findings demonstrate the importance of both diluent and test length on detection threshold values measured by a single staircase procedure and emphasize the need for standardization of procedures for threshold testing.