The polyether bistratene A activates protein kinase C-delta and induces growth arrest in HL60 cells

Biochem Biophys Res Commun. 1996 May 24;222(3):802-8. doi: 10.1006/bbrc.1996.0830.

Abstract

Bistratene A (BisA) induced growth arrest in G2/M in HL60 cells. In addition, BisA-treated cells (50 nM for 48 h) became adherent and expressed the adhesion molecule CD11c, but did not express the monocyte enzyme alpha-napthyl acetate esterase or phagocytose complement coated yeasts. BisA activated protein kinase C (PKC)-delta and induced translocation of PKC-delta to the nucleus. This suggests that activation of PKC-delta can induce growth arrest and cell adhesion, but is insufficient to mediate full differentiation of HL60 cells. BisA has potential as a new probe for determining the function of PKC isoenzymes, specifically PKC-delta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetamides*
  • Cell Adhesion / drug effects
  • Cell Compartmentation / drug effects
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Enzyme Activation / drug effects
  • Ethers, Cyclic / pharmacology*
  • Fluorescent Antibody Technique, Indirect
  • Growth Inhibitors / pharmacology*
  • HL-60 Cells
  • Humans
  • Isoenzymes / metabolism*
  • Phosphorylation
  • Protein Kinase C / metabolism*
  • Protein Kinase C-delta
  • Pyrans*
  • Spiro Compounds

Substances

  • Acetamides
  • Ethers, Cyclic
  • Growth Inhibitors
  • Isoenzymes
  • Pyrans
  • Spiro Compounds
  • bistratene A
  • PRKCD protein, human
  • Protein Kinase C
  • Protein Kinase C-delta