Incidence of human papilloma virus in patients with invasive cervical carcinoma

R C Sobti; N Capalash; S Sehgal; B D Gupta; P Sharma

doi:10.1016/0165-4608(95)00344-4

Incidence of human papilloma virus in patients with invasive cervical carcinoma

Cancer Genet Cytogenet. 1996 Jun;88(2):175-80. doi: 10.1016/0165-4608(95)00344-4.

Authors

R C Sobti¹, N Capalash, S Sehgal, B D Gupta, P Sharma

Affiliation

¹ Department of Biotechnology, Panjab University, Chandigarh, India.

PMID: 8640731
DOI: 10.1016/0165-4608(95)00344-4

Abstract

The dot-blot hybridization of biotin-dUTP-labeled HPV-16 DNA with genomic DNA extracted from biopsies taken from patients with invasive carcinoma and abnormal cytology showed the presence of HPV-DNA in 88% and 80% cases under relaxed conditions and 40% and 20% cases under stringent conditions of hybridization, respectively. Southern blot revealed the HPV-DNA in randomly integrated form in two cases and in episomal form in the other two.

MeSH terms

Blotting, Southern
DNA, Viral / isolation & purification*
Female
Humans
Papillomaviridae / genetics
Papillomaviridae / isolation & purification*
Uterine Cervical Neoplasms / virology*

Substances

DNA, Viral