Cloning of a putative ligand for the T1/ST2 receptor

J Biol Chem. 1996 Mar 8;271(10):5784-9. doi: 10.1074/jbc.271.10.5784.

Abstract

T1/ST2 is a receptor-like molecule homologous to the type I interleukin-1 receptor. Despite this sequence similarity, we have been unable to demonstrate binding of T1/ST2 to any of the three interleukin-1 species. In searching for a ligand for T1/ST2, we have cloned a cell surface protein to which it binds. This protein is unable to initiate signal transduction by the T1/ST2 receptor in several in vitro assays.

Publication types

  • Comparative Study

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA / metabolism
  • Female
  • Humans
  • Interleukin-1 Receptor-Like 1 Protein
  • Interleukin-8 / genetics
  • Kinetics
  • Membrane Proteins*
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Organ Specificity
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • Proteins / metabolism*
  • Rats
  • Receptors, Cell Surface
  • Receptors, Interleukin
  • Receptors, Interleukin-1 / biosynthesis
  • Receptors, Interleukin-1 / chemistry
  • Receptors, Interleukin-1 / metabolism*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Tumor Cells, Cultured

Substances

  • IL1RL1 protein, human
  • Il1rl1 protein, mouse
  • Interleukin-1 Receptor-Like 1 Protein
  • Interleukin-8
  • Membrane Proteins
  • NF-kappa B
  • Proteins
  • Receptors, Cell Surface
  • Receptors, Interleukin
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • DNA

Associated data

  • GENBANK/U41804
  • GENBANK/U41805