Abstract
Expression of a pykF-lacZ fusion was studied as a function of the carbon source in wild-type strains and strains lacking or overproducing the FruR protein of Escherichia coli. FruR controls the response to the carbon source by repressing pykF expression more strongly under gluconeogenic than under glycolytic conditions, a phenomenon we term catabolite activation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / physiology*
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Base Sequence
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Enzyme Activation
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial / physiology*
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Gluconeogenesis
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Glycolysis
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics
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Pyruvate Kinase / genetics*
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Recombinant Fusion Proteins / biosynthesis
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Repressor Proteins / biosynthesis
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Repressor Proteins / physiology*
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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FruR protein, E coli
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Recombinant Fusion Proteins
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Repressor Proteins
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FruR protein, Bacteria
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Pyruvate Kinase
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beta-Galactosidase