Abstract
Parallel simultaneous synthesis of fifty linear peptides has been carried out in order to compare in detail two promising methodologies of simultaneous multiple peptide synthesis (SMPS): the "T bag" method, utilizing 4-methyl-benzhydrylamine resin (MeBHA), and synthesis on derivatized Fmoc-Gly-O-cotton fabric strips. The basic set of experiments, which utilizes identical Fmoc/Bu(t) strategy for both approaches, shows that the peptides synthesized on cotton are superior in purity to those synthesized using T bags. In experiments utilizing Boc/Bzl strategy in T bags, the purities of peptides were higher than in the case of peptides synthesized in T bags by Fmoc/Bu(t) strategy, and comparable with the purities achieved in synthesis performed on cotton. The lower yields on cotton are caused by mechanical losses in the given experimental arrangement.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / chemistry
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Benzhydryl Compounds / chemistry
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Calcitonin / chemistry
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Fluorenes / chemistry
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Formic Acid Esters / chemistry
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Gastrins / chemistry
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Gossypium / chemistry
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Insect Hormones / chemistry
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Melanocyte-Stimulating Hormones / chemistry
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Molecular Sequence Data
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Neuropeptides / chemistry
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Oligopeptides / chemical synthesis*
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Oligopeptides / chemistry
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Oligopeptides / isolation & purification
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Peptides / chemistry
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Pyrrolidonecarboxylic Acid / analogs & derivatives
Substances
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4-methylbenzhydrylamine resin
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Amino Acids
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Benzhydryl Compounds
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Fluorenes
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Formic Acid Esters
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Gastrins
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Insect Hormones
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N(alpha)-fluorenylmethyloxycarbonylamino acids
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Neuropeptides
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Oligopeptides
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Peptides
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adipokinetic hormone
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t-butyloxycarbonyl group
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allatostatin
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alytesin
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Melanocyte-Stimulating Hormones
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Calcitonin
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Pyrrolidonecarboxylic Acid