Production of polyclonal antibodies against 1,1'-ethyliden bis[L-tryptophan] (EBT), a potential contaminant causing eosinophilia-myalgia syndrome (EMS)

N Kurihara; H Yanagisawa; Z Jin; O Wada

doi:10.1016/0378-4274(93)90003-g

Production of polyclonal antibodies against 1,1'-ethyliden bis[L-tryptophan] (EBT), a potential contaminant causing eosinophilia-myalgia syndrome (EMS)

Toxicol Lett. 1993 Mar;66(3):231-6. doi: 10.1016/0378-4274(93)90003-g.

Authors

N Kurihara¹, H Yanagisawa, Z Jin, O Wada

Affiliation

¹ Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.

PMID: 8475503
DOI: 10.1016/0378-4274(93)90003-g

Abstract

Eosinophilia-myalgia syndrome (EMS) is thought to be caused by the intake of contaminated L-tryptophan products. 1,1'-Ethyliden bis[L-tryptophan] (EBT) is one of the contaminants in the L-tryptophan products and is thought to be a possible cause of EMS. In the present study we report that anti-EBT antibodies are generated by immunizing rabbits with the low molecular weight substance, EBT (molecular weight 434). These antibodies may be available tools for elucidating the mechanisms responsible for the outbreak and/or development of EMS.

MeSH terms

Animals
Antibody Formation*
Binding Sites
Eosinophilia-Myalgia Syndrome / chemically induced*
Immunization
Male
Molecular Weight
Rabbits
Serum Albumin, Bovine
Spectrophotometry, Ultraviolet
Tryptophan / analogs & derivatives*
Tryptophan / immunology
Tryptophan / toxicity

Substances

1,1'-ethylidene bis(tryptophan)
Serum Albumin, Bovine
Tryptophan