Frictional transition of pesticides, the transition of the dried pesticide due to rubbing, from protective clothing was investigated by using an AATCC standard crockmeter. One insecticide (carbaryl) and two herbicides (atrazine and metolachlor) were studied, together with three protective clothing materials [cotton, polyester, and polyester/cotton (65/35) blend] and three crock fabrics (cotton, nylon, and silk). The effects of the properties of pesticide, fabrics, water, and perspiration on the frictional transition are discussed. The experiment showed that up to 12% of the pesticide could transfer from contaminated protective clothing to the skin through rubbing. Choosing the right materials for protective clothing and underwear would greatly decrease the frictional transition of the pesticide.