Abstract
A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C18 gel followed by reversed-phase chromatography using a biocompatible PepRPC HR 5/5 column with a pharmacia fast protein liquid chromatographic system. This is a very effective method for isolating and fractionating iturin A and surfactin, two lipopeptides of different nature, co-produced by Bacillus subtilis strain S499. The presence of homologous lipopeptides was easily detected.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Amino Acids / analysis
-
Anti-Bacterial Agents*
-
Antifungal Agents / chemistry
-
Antifungal Agents / isolation & purification*
-
Antifungal Agents / pharmacology
-
Bacillus subtilis / metabolism
-
Bacterial Proteins / chemistry
-
Bacterial Proteins / isolation & purification*
-
Bacterial Proteins / pharmacology
-
Chromatography, Gel
-
Chromatography, Liquid / methods*
-
Chromatography, Thin Layer
-
Lipopeptides
-
Lipoproteins / chemistry
-
Lipoproteins / isolation & purification*
-
Lipoproteins / pharmacology
-
Molecular Sequence Data
-
Peptides*
-
Peptides, Cyclic / chemistry
-
Peptides, Cyclic / isolation & purification
-
Peptides, Cyclic / pharmacology
-
Spectrophotometry, Ultraviolet
Substances
-
Amino Acids
-
Anti-Bacterial Agents
-
Antifungal Agents
-
Bacterial Proteins
-
Lipopeptides
-
Lipoproteins
-
Peptides
-
Peptides, Cyclic
-
surfactin peptide
-
iturin A