Abstract
We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classical glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blastocyst / enzymology*
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Blotting, Northern
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DNA
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Female
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Gene Expression Regulation, Enzymologic*
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Gene Library
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Glutathione Peroxidase / biosynthesis*
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Glutathione Peroxidase / genetics*
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Liver / drug effects
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Liver / enzymology*
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Male
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Molecular Sequence Data
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Myocardium / enzymology
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Oligonucleotide Probes
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Phospholipid Hydroperoxide Glutathione Peroxidase
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RNA, Messenger / metabolism*
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Rats
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Selenium / deficiency
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Selenium / metabolism*
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Selenium / pharmacology
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Sequence Homology, Nucleic Acid
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Swine / genetics*
Substances
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Oligonucleotide Probes
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RNA, Messenger
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DNA
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Phospholipid Hydroperoxide Glutathione Peroxidase
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Glutathione Peroxidase
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Selenium
Associated data
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GENBANK/L12743
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GENBANK/S63833
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GENBANK/S63834
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GENBANK/S63835
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GENBANK/S63836
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GENBANK/S63837
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GENBANK/S63838
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GENBANK/U11085
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GENBANK/U11086
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GENBANK/U11087