Abstract
Proteins and peptides separated by polyacrylamide gel electrophoresis either in acetic acid-urea or in sodium dodecyl sulfate (SDS)-containing gels were electroblotted onto carboxymethylcellulose membrane and eluted from the membrane by dilute acid. Polypeptides thus recovered with a high yield can be used to get structural or biological data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Carboxymethylcellulose Sodium*
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Cell Nucleus / chemistry
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Electrochemistry
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Electrophoresis, Polyacrylamide Gel
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Histones / chemistry
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Histones / isolation & purification
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Humans
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Male
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Membranes, Artificial
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Molecular Sequence Data
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Protamines / chemistry
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Proteins / chemistry
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Proteins / isolation & purification*
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Ribonuclease, Pancreatic / chemistry
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Ribonuclease, Pancreatic / isolation & purification
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Sequence Analysis*
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Serum Albumin, Bovine / chemistry
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Serum Albumin, Bovine / isolation & purification
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Spermatozoa / chemistry
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Spermatozoa / ultrastructure
Substances
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Histones
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Membranes, Artificial
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Protamines
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Proteins
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protamine P1
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protamine P2
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Serum Albumin, Bovine
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Ribonuclease, Pancreatic
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Carboxymethylcellulose Sodium