G-banding and karyotype analyses of cells in seventeen passages of SO-Rb50 during a long period of culture for about four years were performed. Three chromosome markers 13q14-, 1p36+ and 12p13+ were found. Cells possessed 13q14- reduced to zero after the 200th passage while 1p+ and 12p+ cells increased to 100% after 30 and 200 passages respectively. Abnormal chromosomes, ring chromosomes, chromosome radiuses and double minutes were also observed. These chromosomal changes were more often seen before the 200th passage. The significance of these changes are discussed.