Abstract
Adenovirus E1A proteins inhibit expression of the collagenase gene but activate expression of the c-jun gene. Both effects are mediated by TPA-responsive elements (TREs), the binding sites for members of the AP-1 transcription factor family. By a process that is independent of the retinoblastoma gene product, E1A distinguishes between different AP-1 factors: in vivo binding of Jun/Jun homodimers and Jun/Fos heterodimers to the collagenase TRE is totally blocked by E1A while, in contrast, there is no inhibition of Jun/ATF-2 binding to the TRE sequences in the c-jun promoter. Altered phosphorylation of the DNA binding domain of cJun is not involved in the inhibition of cJun/cJun and cJun/cFos binding. E1A does, however, cause hyperphosphorylation of the transactivation domain of cJun, which is likely to be responsible for the enhanced c-jun transcription by E1A mediated through cJun/ATF-2 heterodimers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activating Transcription Factors
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Adenovirus E1A Proteins / biosynthesis
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Adenovirus E1A Proteins / metabolism*
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Adenoviruses, Human / genetics*
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Base Sequence
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Blood Proteins / metabolism
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Cell Line, Transformed
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Cells, Cultured
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Collagenases / biosynthesis
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Collagenases / genetics
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DNA Primers
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Embryo, Mammalian
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Viral*
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Genes, jun*
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Peptide Fragments / isolation & purification
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Phosphorylation
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Proto-Oncogene Proteins c-jun / biosynthesis
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Proto-Oncogene Proteins c-jun / metabolism*
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Recombinant Fusion Proteins / metabolism
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Retina
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Simian virus 40 / genetics
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factors / metabolism
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Transcription, Genetic
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Transcriptional Activation
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Transfection
Substances
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Activating Transcription Factors
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Adenovirus E1A Proteins
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Blood Proteins
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DNA Primers
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Macromolecular Substances
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Peptide Fragments
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Proto-Oncogene Proteins c-jun
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Recombinant Fusion Proteins
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Transcription Factors
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Collagenases
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Tetradecanoylphorbol Acetate