PAF activation of a voltage-gated R-type Ca2+ channel in human and canine aortic endothelial cells

Br J Pharmacol. 1993 Oct;110(2):519-20. doi: 10.1111/j.1476-5381.1993.tb13841.x.

Abstract

By the use of fura-2 and digital imaging techniques, [K]o depolarization or PAF (10(-9) M) were shown to induce a sustained increase of [Ca]i in human or canine single aortic vascular endothelial cells (VEC) that was insensitive to nifedipine but sensitive to (-)-PN200-110 or to lowering of [Ca]o. The PAF-induced effect on [Ca]i was blocked by the PAF receptor antagonist, WEB2170. Our results suggest that [K]o depolarization and PAF increase [Ca]i via the activation of R-type Ca2+ channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azepines / pharmacology
  • Calcium Channel Blockers / pharmacology
  • Calcium Channels / metabolism*
  • Calcium Channels / physiology
  • Electrophysiology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Fura-2
  • Humans
  • Image Processing, Computer-Assisted
  • In Vitro Techniques
  • Ion Channel Gating / physiology*
  • Isradipine / pharmacology
  • Male
  • Platelet Activating Factor / antagonists & inhibitors
  • Platelet Activating Factor / metabolism*
  • Potassium / pharmacology
  • Triazoles / pharmacology

Substances

  • Azepines
  • Calcium Channel Blockers
  • Calcium Channels
  • Platelet Activating Factor
  • Triazoles
  • bepafant
  • Potassium
  • Fura-2
  • Isradipine