Abstract
The tumor promoter okadaic acid is a potent inhibitor of the serine/threonine protein phosphatases 1 and 2A. Addition of okadaic acid to v-Src-transformed BALB/c 3T3 cells reverted them to a flat morphology, increased fibronectin levels in the extracellular matrix, reduced saturation density, and inhibited the formation of colonies in soft agar. The ability of v-Src-transformed cells to proliferate in low serum was also inhibited by okadaic acid. These data implicate serine/threonine phosphatases in v-Src-induced transformation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Cell Adhesion / physiology
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Cell Division
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Cell Transformation, Neoplastic / drug effects*
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Viral / drug effects*
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Cell Transformation, Viral / genetics
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Ethers, Cyclic / pharmacology*
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Genes, src*
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Mice
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Mice, Inbred BALB C
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Okadaic Acid
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Phosphoprotein Phosphatases / antagonists & inhibitors*
Substances
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Ethers, Cyclic
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Okadaic Acid
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Phosphoprotein Phosphatases