A comparative study on growth in soft-agar, adherence to glass and haemolysis types of coagulase-negative staphylococci

Acta Microbiol Hung. 1993;40(3):181-9.

Abstract

Growth properties of coagulase-negative staphylococci in the presence and in the absence of human and rabbit serum in soft-agar prepared in modified Staphylococcus 110 broth were studied. The adherent growth was examined in modified Staphylococcus 110 broth and 1% glucose-meat broth. Of 100 strains examined 69% exhibited diffuse, 18% compact, 7% transient and 6% mixed growth. Compact type colonies were mainly characteristic of Staphylococcus haemolyticus strains. The presence of serum failed to influence the types of colony morphology in any of the strains. Sixty-three percent of the strains showed adherent growth; none of the S. haemolyticus strains produced adherent growth. The glucose-meat broth, unlike modified Staphylococcus 110 broth, was suitable to study adherence. The coincidence of the compact colony morphology in soft-agar and the absence of adherent growth seems to be a taxonomic sign for the species S. haemolyticus and differentiate it from the species Staphylococcus epidermidis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agar
  • Bacterial Adhesion
  • Coagulase / metabolism
  • Culture Media
  • Glass
  • Hemolysis
  • Humans
  • Species Specificity
  • Staphylococcus / classification
  • Staphylococcus / growth & development*
  • Staphylococcus / physiology
  • Staphylococcus epidermidis / classification
  • Staphylococcus epidermidis / growth & development
  • Staphylococcus epidermidis / physiology

Substances

  • Coagulase
  • Culture Media
  • Agar