[Dig-DNA probe for etiologic diagnosis in tuberculosis patients]

Zhonghua Jie He He Hu Xi Za Zhi. 1993 Aug;16(4):228-9, 253.
[Article in Chinese]

Abstract

We have detected the sputa DNA with nonradioactive probe which is labelled to the specific sequence DNA, 31bp-188bp, by dig-11-dUTP. It showed that both the Sensitivity and specificity are satisfactory. The positive rate of the 80 specimens are 56.25%, and the 30 positive specimens of sputum culture are all positive when detected by the Dig-DNA probe.

Publication types

  • English Abstract

MeSH terms

  • Base Sequence
  • DNA Probes
  • DNA, Bacterial / analysis*
  • Humans
  • Molecular Sequence Data
  • Mycobacterium tuberculosis / isolation & purification*
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology

Substances

  • DNA Probes
  • DNA, Bacterial