Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

Parasitol Res. 1994;80(1):1-9. doi: 10.1007/BF00932616.

Abstract

Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (> 95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Centrifugation
  • Filtration
  • Lung / microbiology
  • Magnetics
  • Male
  • Mycology / methods
  • Pneumocystis / growth & development*
  • Pneumocystis / isolation & purification*
  • Pneumonia, Pneumocystis / microbiology*
  • Rats
  • Rats, Sprague-Dawley