Abstract
The regulatory sequences of Azospirillum brasilense Sp7 nifH gene were fused with the cam reporter gene and used for studying the factors controlling nifH transcription. A DNA sequence, downstream the ATG codon of nifH, that could be involved in the negative regulation of nifH transcription, was identified. The effect of 1 and 2 mM of ammonium on the transcription of the A. brasilense nifH gene and on the nitrogenase activity, in the presence of the Klebsiella pneumoniae NifA protein, was examined.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azospirillum brasilense / genetics*
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Azospirillum brasilense / metabolism
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology*
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Conjugation, Genetic
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Gene Expression Regulation, Bacterial / genetics*
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Genes, Bacterial*
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Klebsiella pneumoniae / genetics
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Nitrogen Fixation / genetics*
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Nitrogenase / analysis
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Oxidoreductases*
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Plasmids / genetics
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Promoter Regions, Genetic
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Transcription Factors / physiology*
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Transcription, Genetic / genetics*
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Transformation, Bacterial
Substances
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Bacterial Proteins
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NifA protein, Bacteria
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Transcription Factors
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Oxidoreductases
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Nitrogenase
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nitrogenase reductase