Control of nifH transcription in Azospirillum brasilense: involvement of NifA and of cis-acting sequences

FEMS Microbiol Lett. 1994 Jan 1;115(1):57-62. doi: 10.1111/j.1574-6968.1994.tb06614.x.

Abstract

The regulatory sequences of Azospirillum brasilense Sp7 nifH gene were fused with the cam reporter gene and used for studying the factors controlling nifH transcription. A DNA sequence, downstream the ATG codon of nifH, that could be involved in the negative regulation of nifH transcription, was identified. The effect of 1 and 2 mM of ammonium on the transcription of the A. brasilense nifH gene and on the nitrogenase activity, in the presence of the Klebsiella pneumoniae NifA protein, was examined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azospirillum brasilense / genetics*
  • Azospirillum brasilense / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / physiology*
  • Conjugation, Genetic
  • Gene Expression Regulation, Bacterial / genetics*
  • Genes, Bacterial*
  • Klebsiella pneumoniae / genetics
  • Nitrogen Fixation / genetics*
  • Nitrogenase / analysis
  • Oxidoreductases*
  • Plasmids / genetics
  • Promoter Regions, Genetic
  • Transcription Factors / physiology*
  • Transcription, Genetic / genetics*
  • Transformation, Bacterial

Substances

  • Bacterial Proteins
  • NifA protein, Bacteria
  • Transcription Factors
  • Oxidoreductases
  • Nitrogenase
  • nitrogenase reductase