Effect of protein kinase C activators on the uptake of GABA by rat brain synaptosomes

Int J Neurosci. 1993 Mar-Apr;69(1-4):131-6. doi: 10.3109/00207459309003323.

Abstract

Synaptosomes were prepared from rat brain by a discontinuous Ficoll gradient method and used for studying the uptake of labelled GABA. Two GABA uptake components were evidenced, a high (Km = 3.13 microM) and a low (Km = 92.4 microM) affinity one. Preincubation of synaptosomes with two different activators of protein kinase C, phorbol 12, 13-diacetate (PDAc) and oleyl-acetyl glycerol (OAG), resulted in a change of GABA uptake. In particular, the low affinity component increased its Vmax by 58-74%, with no change in the Km. No statistically significant modification was detected for the high affinity component.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Culture Techniques
  • Diglycerides / pharmacology*
  • Male
  • Phorbol Esters / pharmacology*
  • Phosphorylation
  • Protein Kinase C / metabolism*
  • Rats
  • Rats, Sprague-Dawley*
  • Synaptosomes / drug effects*
  • gamma-Aminobutyric Acid / metabolism*

Substances

  • Diglycerides
  • Phorbol Esters
  • phorbol-12,13-diacetate
  • gamma-Aminobutyric Acid
  • 1-oleoyl-2-acetylglycerol
  • Protein Kinase C