Employment of fast-protein liquid chromatography (FPLC) system and a high-pressure filtration cell is described for the production of liposomes by the rapid extrusion technique. The system allows processing of up to 50 ml liposomal suspension in one batch at a defined temperature and applied pressure of up to 4.5 MPa. This relatively simple technique is applicable in any laboratory equipped with FPLC or HPLC systems.